Publication List

Journal Paper Publications (Peer-reviewed)

Kontomaris, S.V.; I. Psychogios, Malamou, A.; Stylianou, A. (2025) “Monitoring the Distance and Velocity of Protons in a Medium for Biomedical Applications Using a Straightforward Mathematical Approach”, Sci 2025, 7(2), 49; https://doi.org/10.3390/sci7020049
Pafitani, S., Zachari, L.C., Stylianou, A., Gkretsi, V. (2025) “In vitro models: Can they unreavel the complexities of cancer cell metastasis?”, Biochemica et Biophysica Acta (BBA)-Reviews on cancer, 1880 (2), 189293, https://doi.org/10.1016/j.bbcan.2025.189293
Kontomaris S.V., Malamou A, Stylianou A, (2025) “Simplifying Data Processing in AFM Nanoindentation Experiments on Thin Samples” Eng, 6(2), 32; https://doi.org/10.3390/eng6020032
Petrou, M. and Stylianou, A. (2025) “Open-Source Tools for Assessing Cytoskeleton Properties in Pathological Conditions from Microscopy Images: An Application Note”, Applied Research, (accepted)
Karagkouni, A.C., Polemidiotou, K., Gkretsi, V. and Stylianou, A. (2025) “Atomic force microscopy reveals the influence of substrate collagen concentration and TGF-β on lung fibroblast mechanics”, Micron, 189, 103751, https://doi.org/10.1016/j.micron.2024.103751
Kotsoni, A., Kozaki, L.V., Stylianou, A., Gkretsi, V. (2025) “Interdependent roles for growth differentiation factor-15 (GDF15) and LIMS1 in regulating cell migration: Implications for colorectal cancer metastasis”, Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1872 (3), 119904, https://doi.org/10.1016/j.bbamcr.2025.119904
Stylianou, A., Kontomaris S.V., Polemidiotou, K., Zachariades, A. (2025) “Utilizing collagen-coated hydrogels with defined stiffness as calibration standards for AFM experiments on soft biological materials: the case of lung cells and tissue”, Methods in Microscopy, 1 (2), 151-162, https://doi.org/10.1515/mim-2024-0014
Kontomaris S.V., Malamou A, Stylianou A, (2025) “Development of an accurate simplified approach for data processing in AFM indentation experiments”, Micron, 190, 103782, https://doi.org/10.1016/j.micron.2024.103782
Polemidiotou, K., Kulkarni, S.G., Szydlak, R., Lekka, M., Radmacher, M., Gkretsi, V., Stylianopoulos, T., and Stylianou, A. (2024) “Sarcoma cells remodel their cytoskeleton in order to enhance their invasion and migration”, International Journal of Biological Macromolecules, 282 (1), 136770, https://doi.org/10.1016/j.ijbiomac.2024.136770
Kontomaris S.V., Malamou A, Stylianou A (2024) “Accurate Modelling of AFM Force-Indentation Curves with Blunted Indenters at Small Indentation Depths”, Micromachines, 15 (10), 1209, https://doi.org/10.3390/mi15101209
Kontomaris S.V., Malamou A, Stylianou A, Chliveros G. (2024) “Towards Simpler Modelling Expressions for the Mechanical Characterization of Soft Materials”, Micro and Nanosystems, 16 (3), 172 – 182, 10.2174/0118764029305681240530071757, (link: https://benthamscience.com/article/140839)
Kontomaris, S.V., Malamou, A., Zachariades, A., Stylianou, A. (2024) “A Linear Fit for Atomic Force Microscopy Nanoindentation Experiments on Soft Samples”, Processes, 12 (4), 843, https://doi.org/10.3390/pr12040843
Panagi, M., Mpekris, F., Voutouri, C., Hadjigeorgiou, A.G., Symeonidou, C., Porfyriou,E., Michael, C., Stylianou, A., Martin, D.J., Cabral, H., Constantinidou, A., Stylianopoulos,T. (2024) “Stabilizing Tumor-Resident Mast Cells Restores T-Cell Infiltration and Sensitizes Sarcomas to PD-L1 Inhibition”, Clinical Cancer Research, 2024;30:1–16, https://doi.org/10.1158/1078-0432.CCR-24-0246
Miler, I., Rabasovic, M.D., Askrabic, S., Stylianou, A., Korac, B., Korac, A. (2024) “Short-Term L-Arginine Treatment Mitigates Early Damage of Dermal Collagen Induced by Diabetes”, Bioengineering, 11 (4), 407, https://doi.org/10.3390/bioengineering11040407
Chapalo, I., Stylianou, A., Mégret, P., Theodosiou, A. (2024) “Advances in Optical Fiber Speckle Sensing: A Comprehensive Review”, Photonics, 11 (4), 299, https://doi.org/10.3390/photonics11040299
Christou, C., Stylianou, A., Gkretsi, V. (2024) “Midkine (MDK) in Hepatocellular Carcinoma: More than a Biomarker”, Cells, 13 (2), 136, https://doi.org/10.3390/cells13020136
Kontomaris, S.V., Stylianou, A., Chliveros, G., Malamou, A. (2023) “Overcoming Challenges and Limitations Regarding the Atomic Force Microscopy Imaging and Mechanical Characterization of Nanofibers”, Fibers, 11 (10), 83, https://doi.org/3390/fib11100083
Sarantelli, E., Mourkakis, A., Zacharia, L.C., Stylianou, A., Gkretsi, V. (2023) “Fascin-1 in cancer cell metastasis: old target-new insights”, International Journal of Molecular Sciences, 8;24(14):11253. https://doi.org/10.3390/ijms241411253
Kapnisis, K.; Stylianou, A., Kokkinidou, D., Martin, A., Wang, D., Anderson, P., Prokopi, M., Papastephanou, C., Brott, B., Lemons, J., Anayiotos, A., (2023) “Multi-Level Assessment of Stent-Induced Inflammation in the Adjacent Vascular Tissue”, ACS Biomaterials Science & Engineering, 9, 8, 4747–4760 https://doi.org/10.1021/acsbiomaterials.3c00540
Kontomaris, S.V., Stylianou, A., Chliveros, G., Malamou, A. (2023) “AFM indentation on highly heterogeneous materials using different indenter geometries”, Applied Mechanics, 4(2), 460-475; https://doi.org/10.3390/applmech4020026
Stylianou, A., Voutouri, C., Mpekris, F. Stylianopoulos T. (2023), “Pancreatic Cancer Presents Distinct Nanomechanical Properties During Progression”, Annals of Biomedical Engineering, 51, 602–1615, https://doi.org/10.1007/s10439-023-03168-3
Kontomaris, S.V., Stylianou, A., Georgakopoulos, A., Malamou, A. (2023) “3D AFM nanomechanical characterization of biological materials”, Nanomaterials, 13(3), 395; https://doi.org/10.3390/nano13030395
Kontomaris, S.V., Stylianou, A., Chliveros, G., Malamou, A. (2023) “Determining spatial variability of elastic properties for biological samples using AFM”, Micromachines, 14(1), 182; https://doi.org/10.3390/mi14010182
Kontomaris, S.V., Stylianou, A., Georgakopoulos, A., Malamou, A. (2023) “Is it mathematically correct to fit AFM data (obtained on biological materials) to equations arising from Hertzian mechanics?”, Micron, 2023, 164, 103384, , https://doi.org/10.1016/j.micron.2022.103384
Stylianou, A., Mpekris, F., Voutouri, C., Papoui, A., Constantinidou, A., Kitiris, E., Kailides, M., Stylianopoulos T. (2022) “Nanomechanical properties of solid tumors as treatment monitoring biomarkers”, Acta Biomaterial,154, 324-334, https://doi.org/10.1016/j.actbio.2022.10.021
Panagi, M., Mpekris, F., Chen, P., Voutouri, C., Nakagawa, Y., Martin, J, Hiroi, T., Hashimoto, H., Demetriou, P., Pierides, C., Samuel, R., Stylianou, A., Michael, C., Fukushima, S., Georgiou, P., Papageorgis, P., Papaphilippou, P., Koumas, L., Costeas, P., Ishii, G., Kojima, M., Kataoka, K., Cabral, H., Stylianopoulos, T. (2022) “Polymeric micelles effectively reprogram the tumor microenvironment to potentiate nano-immunotherapy in mouse breast cancer models”, Nature Communications, 13 (1), 7165, https://doi.org/10.1038/s41467-022-34744-1
Kontomaris, S.V., Stylianou, A., Malamou, A. (2022) “Atomic Force Microscopy Nanoindentation Method on Collagen Fibrils”, Materials, 15 (7), 2477, https://doi.org/10.3390/ma15072477
Stylianou, A, (2022) “Assessing Collagen D-band periodicity with Atomic Force Microscopy”, Materials, 15 (4), 1608, https://doi.org/10.3390/ma15041608
Kontomaris, S.V., Stylianou, A., Malamou, A. (2022) “Is it possible to directly determine the radius of a spherical indenter using force-indentation data on soft samples?”, Scanning, 2022, 6463063, org/10.1155/2022/6463063
Kontomaris, S.V., Malamou, A., Stylianou, A. (2022) “The Hertzian theory in AFM nanoindentation experiments regarding biological samples: Overcoming limitations in data processing”, Micron, 155, 103228, org/10.1016/j.micron.2022.103228
Voutouri C., Panagi, M., Mpekris, F., Stylianou, A., Michael, C., Averkiou, M.A., Martin, J.D. and Stylianopoulos, T., (2021) “Endothelin inhibition potentiates cancer immunotherapy revealing mechanical biomarkers predictive of response”, Advanced Therapeutics, 2000289, org/10.1002/adtp.202000289
Stavrou, M., Papaparaskeva, G., Stathis, A., Stylianou, A., Turcu, R., Krasia-Christoforou, T. and Couri, S. (2021)”Synthesis, Characterization and Nonlinear Optical Response of Polyelectrolyte-Stabilised Copper Hydroxide and Copper Oxide Colloidal Nanohybrids”, Optical Materials, 119, 111329, org/10.1016/j.optmat.2021.111329
Kontomaris, S.V., Georgakopoulos, A., Malamou, A., Stylianou, A (2021) “The average Young’s modulus as a physical quantity for describing the depth-dependent mechanical properties of cells”, Mechanics of Materials, 158, 103846, org/10.1016/j.mechmat.2021.103846
Miranda, A. Gómez-Varela, A., Stylianou, A., Hirvonen, L., Sánchez, H., De Beule, P. (2021) “How did Correlative Atomic Force Microscopy and Super-Resolution Microscopy evolve in the quest for unravelling enigmas in biology? “, Nanoscale , 13, 2082-2099, org/10.1039/D0NR07203F
Panagi, M., Voutouri, C., Mpekris, F., Papageorgis,P., Martin, M., Martin, J. Demetriou, P., Pierides, C., Costeas, P., Polydorou,C., Stylianou, A, Louca, M., Kataoka, K., Cabral, H., Stylianopoulos, T. (2020) “TGF-β inhibition combined with cytotoxic nanomedicine normalizes triple negative breast cancer microenvironment towards anti-tumor immunity “, Theranostics, 10(4), 1910-1922,org/10.7150/thno.36936.
Gkretsi, V., Stylianou, A., Kalli, M. Louca, M., Voutouri, C., Zaravinos, A., Stylianopoulos, T. (2020) ” Silencing of growth differentiation factor-15 promotes breast cancer cell invasion by down-regulating focal adhesion genes”, Anticancer Research, 40(3): 1375-1385, org/10.21873/anticanres.14079
Kontomaris, S.V., Malamou, A., Stylianou, A (2020) “Is it necessary to calculate Young’s modulus in AFM nanoindentation experiments regarding biological samples?”, Micro and Nanosystems, 12, 1-6 org/10.2174/1876402912666200214123734
Kontomaris, S.V., Stylianou, A., Nikita, K.S. Malamou, A. (2020) “A discussion regarding the application of the Hertz contact theory on biological samples in AFM nanoindentation experiments”, Micro and Nanosystems, 12, 1-7, org/10.2174/1876402912666200115160207
Kontomaris, S.V., Malamou, A., Stylianou, A (2020) “A new approach for the AFM-based mechanical characterization of biological samples”, Scanning, 2020, 2896792. org/10.1155/2020/2896792
Kontomaris, S.V., Stylianou, A., Nikita, K.S. Malamou, A., (2019) “Determination of the linear elastic regime in AFM nanoindentation experiments on cells”, Materials Research Express, 6 (11), 115410, doi:10.1088/2053-1591/ab4f42.
Stylianou, A, Kontomaris, S.V., Alexandratou, E., Grant, C (2019) “Atomic Force Microscopy on biological materials related to pathological conditions”, Scanning, 19, 8452851, org/10.1155/2019/8452851
Kontomaris, S.V., Alexandratou, E., Grant, C, Stylianou, A, (2019) “Atomic Force Microscopy: In Sickness and in Health” Editorial to the Special Issue Atomic Force Microscopy: In Sickness and in Health” Scanning, 19, 6149247, org/10.1155/2019/6149247
Kontomaris, S.V., Stylianou, A., Nikita, K.S., Malamou, A., Stylianopoulos, T (2019) “A simplified approach for the determination of fitting constants in Oliver – Pharrmethod regarding biological samples”, Physical Biology, 16(5):056003. doi: 1088/1478-3975/ab252e.
Stylianou A., Gkretsi, V., Louca, M., Zacharia, L.C., and Stylianopoulos, T. (2019) ” Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts “, Journal of the Royal Society of Interface, 16 (154):20190226, org/10.1098/rsif.2019.0226
Louca, M., Stylianou, A., Minia, A., Pliaka, V., Alexopoulos, G. L., Gkretsi, V. and Stylianopoulos, T. (2019) “Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation”, Scientific Reports, 9 , 7782, org/10.1038/s41598-019-44200-8.
Gkretsi, V., Louca, M., Stylianou, A., Minadakis, G., Spyrou, G.M and Stylianopoulos, T. (2019) “Inhibition of breast cancer cell invasion by Ras suppressor-1 (RSU-1) silencing is reversed by Growth Differentiation Factor-15 (GDF-15)”, International Journal of Molecular Sciences, 20 (1), doi:10.3390/ijms20010163
Stylianou A., Lekka, M. and Stylianopoulos, T. (2018). “AFM Assessing of Nanomechanical FingerPrints for Cancer Early Diagnosis and Classification: from single cell to tissue level”, Nanoscale, 10(45):20930-20945, doi: 10.1039/c8nr06146g
Kontomaris, S.V, Stylianou, A., Malamou, A, Nikita, K.S (2018) “An alternative approach for the Young’s modulus determination of biological samples regarding AFM indentation experiments”, Materials Research Express,6 (2) 025407, org/ 10.1088/2053-1591/aaef10
Stylianou, A., Gkretsi, V. and Stylianopoulos, T. (2018) “Transforming Growth Factor-β modulates Pancreatic Cancer Associated Fibroblasts cell shape, stiffness and invasion”, Biophysica and Biochemica Acta-General Subjects, 1862(7),1537-1546, org/10.1016/j.bbagen.2018.02.009
Stylianou, A., Gkretsi, V. and Stylianopoulos, T. (2018) “Atomic Force Microscopy Nano-Characterization of 3D Collagen Gels with Tunable Stiffness”, MethodsX, 5, 503-513, org/10.1016/j.mex.2018.05.009
Kontomaris, S.V, Stylianou, A., Malamou, A, Stylianopoulos, T. (2018) “A discussion regarding the approximation of cylindrical and spherical shaped samples as half spaces in AFM nanoindentation experiments”, Materials Research Express, 5 (8), org/10.1088/2053-1591/aad2c9
Stylianou, A. (2017) “Atomic Force Microscopy for Collagen-Based Nanobiomaterials”, Journal of Nanomaterials, id. 9234627, doi:10.1155/2017/9234627, (invited)
Kontomaris, S.V and Stylianou, A. (2017) “Atomic Force Microscopy for University Students, Application in Biomaterials” European Journal of Physics, 38 (3), org/10.1088/1361-6404/aa5cd6
Gkretsi, V., Stylianou, A. and Stylianopoulos, T. (2017) ” Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and urokinase-plasminogen activator (uPA)” Experimental Cell Research, 352(2): 281-292, doi: 10.1016/j.yexcr.2017.02.019
Gkretsi, V., Stylianou, A., Louca, M. and Stylianopoulos, T. (2017), “Identification of Ras Suppressor-1 (RSU-1) as a potential breast cancer metastasis biomarker using a three-dimensional in vitro approach” (17) Oncotarget, 8(16):27364-27379, doi: 10.18632/oncotarget.16062
Stylianou, A, Talias, M.A ” Big data in healthcare: a discussion on the big challenges ” (2017), Health and Technology, 7 (1), 97-107, doi:10.1007/s12553-016-0152-4
Stylianou, A. and Stylianopoulos, T. (2016) “Atomic Force Microscopy Probing of Cancer Cells and Tumor Microenvironment Components” BioNanoScience, 6 (1), 33-46, doi:10.1007/s12668-015-0187-4 (invited)
Kontomaris, S.V, Stylianou, A. Yova, D (2016) “Investigation of the mechanical properties of collagen fibrils under the influence of low power red laser irradiation”, ‘Special issue on Emerging Trends in Biomedical Physics and Engineering’, Biomedical Physics and Engineering Express, 2 0640, org/10.1088/2057-1976/aa5195
Kontomaris, S.V., Yova, D., Stylianou, A. and Politopoulos, K. (2015) “The Significance of the Percentage Differences of Young’s Modulus in the AFM Nanoindentation Procedure” Micro and Nanosystems, 7 (2), 86-97, doi: 10.2174/1876402908666151111234441
Gkretsi, V., Stylianou, A., Papageorgis, P., Polydorou, C. and Stylianopoulos, T. (2015) “Remodeling components of the tumor microenvironment to enhance cancer therapy” Frontiers in Oncology, 5 (214), doi: 10.3389/fonc.2015.00214
Stylianou, A., Yova, D., (2015) “Atomic Force Microscopy Investigation of the Interaction of Low-Level Laser Irradiation of Collagen Thin Films in Correlation with Fibroblast Response” Lasers in Medical Science, 30 (9), doi:2369-2379 10.1007/s10103-015-1823-5
Stylianou, A., Kontomaris S.V, Yova D. (2014) “Assessing Collagen Nanoscale Thin Films Heterogeneity by AFM Multimode Imaging and Nanoindetation for NanoBioMedical Applications”, Special Issue on Bio-micro and bio-nano technologies, Micro and Nanosystems, 6 (2), 95-102, doi:10.2174/187640290602141127114448
Kontomaris S.V, Yova D., Stylianou A., Balogiannis G., (2014) ” The effects of UV irradiation on collagen D‐band revealed by atomic force microscopy”, Scanning, 37 (23), 101-111, doi:10.1002/sca.21185
Stylianou, A., Yova, D., Alexandratou, E. (2014) “Investigation of the Influence of UV Irradiation on Collagen Thin Films by AFM Imaging Microscopy”, Materials Science and Engineering: C, Materials for Biological Applications, 45, 455–468, doi: 10.1016/j.msec.2014.09.006
Stylianou, A, Talias, MA (2014) “The ‘magic light’: a discussion on laser ethics”, Science and Engineering Ethics, 21 (4), 979-998, doi: 10.1007/s11948-014-9566-4
Stylianou, A, Talias, MA. (2013) “Nanotechnology-supported THz medical imaging” F1000 Research, 2:100, 2 (1), 1-14, doi: 10.12688/f1000research.2-100.v1
Stylianou, A., Yova, D., Alexandratou, E. (2013) “Nanotopography of collagen thin films in correlation with fibroblast response”, Journal of Nanophotonics, Special Section: Nanostructured Thin Films: Fundamentals and Applications, 7 (1), art no. 073590, doi: 10.1117/1.JNP.7.073590
Stylianou, A., Yova, D. (2013) “Surface Nanoscale Imaging of Collagen Thin by Atomic Force Microscopy”, Materials Science and Engineering: C, Materials for Biological Applications, 33 (5), 2947-2957, doi:10.1016/j.msec.2013.03.029
Stylianou, A., Politopoulos K, Kyriazi M, Yova D. (2011) “Combined information from AFM imaging and SHG signal analysis of collagen thin films”, Biomedical Signal Processing and Control, 6 (3), 307-313, doi:10.1016/j.bspc.2011.02.006

Book chapters

Stylianou, A. Gkretsi, V., Huntosova, V., Theodosiou, A. (2024) “Fluorescence Microscopy of the Cell & Cell Interactions to Study Dynamic Cellular Processes”, In: Walter, A., Slezak, P., Müller, R., Kerckhofs, G. and Bajoghli, B. (eds) Tissue Engineering and Regeneration. Bioimaging in Tissue Engineering and Regeneration, Springer-Nature, https://doi.org/10.1007/978-3-030-85569-7_1-1
Stylianou, A., Gkretsi, V., Walter, A. , Glösmann, M. (2021) “Part I: Ex-vivo Imaging, I.1 – Light and fluorescence microscopy”, In: Walter, A., Mannheim, J., Caruana, C.J. (eds) Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 1, IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology, Hardback ISBN: 9780750330572, Ebook ISBN: 9780750330596 , https://doi.org/10.1088/978-0-7503-3059-6ch1
Miranda, A., Dykas, M., Gómez, A., De Beule, P., Moura, K. and Stylianou, A., (2020) ” Part III: Correlative multimodal imaging and image data fusion, III.1.b Correlative atomic force microscopy”, In: Walter, A., Mannheim, J., Caruana, C.J. (eds) Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 2, IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology, Hardback ISBN: 9780750337458, Ebook ISBN: 9780750337472. https://doi.org/10.1088/978-0-7503-3747-2ch17
Lekka, M., Stylianou, A. and Plochberger, B. (2020) “Part I: Ex-vivo Imaging, I.6 – Atomic force microscopy and spectroscopy”, In: Walter, A., Mannheim, J., Caruana, C.J. (eds) Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 1, IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology, Hardback ISBN: 9780750330572, Ebook ISBN: 9780750330596, https://doi.org/10.1088/978-0-7503-3059-6ch30
Stylianou, A., Gkretsi, V., Patrickios, C.S., Stylianopoulos, T. (2017) “Exploring the nano-surface of collagenous and other fibrotic tissues with AFM” In: Rittié L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627, pp. 453-489, Humana Press, New York, NY, doi: 0.1007/978-1-4939-7113-8_29

Research Publications in Referred Conference Proceedings

Theodosiou, A. and Stylianou, A. (2024) “Adaptive fiber optic fluorescence analysis for cancer tissues via collagen detection”, Proceedings of SPIE, Biomedical Spectroscopy, Microscopy, and Imaging III, 13006, 226-231, https://doi.org/10.1117/12.3014718
Stylianou, A., Voutouri, C., Mpekris, F., Papoui, A., Polemidiotou, K., Stylianopoulos T. (2022) “Solid tumors nanomechanical fingerprints and collagen-based optical signatures”, SPIE, Proceedings Volume 121470I, Tissue Optics and Photonics II, https://doi.org/10.1117/12.2621002
Stylianou, A., Voutouri, C., Mpekris, F., Stylianopoulos T., (2021) “Pancreatic Cancer Collagen-Based Optical Signatures”, Proceedings of SPIE Volume 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics; 1164613, org/10.1117/12.2582590.
Gkretsi, V., Stylianou, A, Stylianopoulos, T. (2016) “Targeting breast cancer metastasis: A bioengineering approach”, International Journal of Molecular Medicine, 38, S1. pp S37.
Stylianou, A., Kontomaris, S.V., Yova, D (2016) “Probing Collagen Nanocharacteristics After Low-Level Red Laser Irradiation” IFMBE Proceedings, 57, 264-268, doi: 10.1007/978-3-319-32703-7_53
Kontomaris, S.V., Yova, D, Sambani, k and Stylianou, A. (2016) ” AFM Investigation of the Influence of Red Light Irradiation on Collagen” IFMBE Proceedings, 57, 269-274, doi:10.1007/978-3-319-32703-7_54
Stylianou, A., Kontomaris, S.V., Yova, D., Balogiannis, G. (2013) “AFM Multimode Imaging and Nanoindetation Method For Assessing Collagen Nanoscale Thin Films Heterogeneity” IFMBE Proceedings, 41, 407-410, doi: 10.1007/978-3-319-00846-2_101
Stylianou, A., Yova, D., Alexandratou, E., Petri, Α. (2013) “Atomic force imaging microscopy investigation of the interaction of ultraviolet radiation with collagen thin films ” Progress in Biomedical Optics and Imaging- Proceedings of SPIE, 8594, art. no. 85940E; doi:10.1117/12.2002460
Stylianou, A.,Yova, D., Politopoulos, K (2012) “Atomic Force Microscopy Surface Nanocharacterization of UV-Irradiated Collagen Thin Films”, IEEE Proceedings, 12^th International Conference on Bioinformatics & Bioengineering (BIBE 2012), art. no. 6399741, pp. 602-607, doi: 10.1109/BIBE.2012.6399741
Kontomaris, S.V., Stylianou, A., Yova, D., Politopoulos, K (2012) “Mechanical Properties of Collagen Fibrils on Thin Films by Atomic Force Microscopy Nanoindentation”, IEEE Proceedings, 12^th International Conference on Bioinformatics & Bioengineering (BIBE 2012), art. no. 6399742, pp. 608-613, doi: 10.1109/BIBE.2012.6399742
Stylianou, A.,Yova, D., Politopoulos, K. (2012) “Atomic Force Microscopy Quantitative and Qualitative Nanoscale Characterization of Collagen Thin Films”, Proceedings of the 5^th Conference on Emerging Technologies in NDT, CRC Press 2012, Chapter 65, 415-420, doi: 10.1201/b11837-75
Stylianou, A., Politopoulos, K., Yova, D. (2011) “Atomic Force Microscopy Imaging of the Nanoscale Assembly of Type I Collagen on Controlled Polystyrene Particles Surfaces” IFMBE Proceedings, 37, pp. 1058-1061, doi: 10.1007/978-3-642-23508-5_275
Stylianou, A., Kontomaris S.B., Kyriazi, M., Yova, D.(2010) “Surface characterization of collagen films by atomic force microscopy” IFMBE Proceedings, 29, pp. 612-615, doi: 10.1007/978-3-642-13039-7_154
Stylianou, A., Kyriazi M, Politopoulos K, Yova D.(2009) “Combined SHG signal information with AFM imaging to assess conformational changes in collagen” IEEE Proceedings, 9^th International Conference on Information Technology and Applications in Biomedicine (ITAB 2009), art. no. 5394311, 1-4, doi: 10.1109/ITAB.2009.5394311

Patents

JD Martin, T Stylianopoulos, F Mpekris, M Panagi, C Voutouri, A Stylianou “Cancer treatment using bosentan in combination with a checkpoint inhibitor” , US Patent App. 18/256,776 (Non-provisional filing on 10/12/2021), https://patents.google.com/patent/US20240024317A1/en

Monographs/Theses

Stylianou, A (2017) “Ethical and Social Aspects of New Medical Technology” (PhD thesis), Open University of Cyprus (ISBN 978-9963-695-61-4)
Stylianou, A (2014) “Optical Radiation Effects on Collagen Topology Studied with AFM and SEM ” (PhD thesis), assessed through Greece National Documentation Center and National Technical University Central Library.
Stylianou, A (2011) “Nanotechnology supported emerging non ionizing medical imaging modalities: The case of OCT and THz” (Master Thesis), The Open University, United Kingdom
Stylianou, A (2007) “Study of the Production of Second Harmonic Generation from Collagen Structures with Atomic Force Microscopy” (Diploma thesis), assessed through National Technical University Central Library (ID. no. 000112848).

Encyclopedia Entries

Entry ID: 21308, Category: Biophysics, Title: Collagen Fibrils, Authors: Andreas Stylianou , Stylianos Vasileios Kontomaris, Created Date: 2 April 2022, Author: Andreas Stylianou, https://encyclopedia.pub/entry/21308
Entry ID: 21307, Category: Biophysics, Title: Collagen D-Band Periodicity, Authors: Andreas Stylianou, Created Date: 2 April 2022, Author: Andreas Stylianou, https://encyclopedia.pub/entry/21307

Conference Presentations (presented by PI)

Stylianou, A (2025) “Nanomechanical biomarkers for non-communicable diseases”, AFM BioMed Conference 2025, 8^th-11^th April 2025, Barcelona, Spain (podium)
Stylianou, A (2024) “AFM-Based nanomechanical biomarkers”, ESB2024, 29^th Congress of the European Society of Biomechanics, 30 June- 3 July, 2024, Edinburgh, Scotland (Perspective Talk)
Stylianou, A (2024) “A linear fit for determining Young’s modulus in very deep spherical indentations”, ESB2024, 29^th Congress of the European Society of Biomechanics, 30 June- 3 July, 2024, Edinburgh, Scotland (poster)
Stylianou, A (2024) “Nanomechanical fingerprints of pulmonary fibrosis”, NANOINBIO 2024, Nanosicences for Life and Materials Sciences, 4^th edition, April 14 -20, Le Gosier, Gouadeloupe, French Caribbean, France (podium)
Stylianou, A., (2023) “AFM-based nanomechanical biomarkers: from cancer to pulmonary fibrosis” EBSA 2023, July 31 – August 4, Stockholm, Sweeden (poster)
Stylianou, A., (2022) “Nanomechanical assessment of solid tumors” 10th International Multithematic Bio-Medical Congress (IMBMC), 4 – 5 November, Nicosia, Cyprus (poster)
Stylianou, A., (2022) “Identifying unique nanomechanical signatures as possible biomarkers”, 1st Βιος-SBSCy Conference, 22-23 October, Nicosia, Cyprus (poster)
Stylianou, A., (2022) “AFM-based nanomechanical biomarkers”, 2022 NanoScientific Forum Europe, 6-7 October, (online, podium)
Stylianou, A., (2022) “MechanoOptical fingerprints as biomarkers for cancer treatment monitoring”, COMULIS Conference Nicosia, 21 / 9/ 2022, Cyprus (podium)
Stylianou, A., Polemdiotou, K., Mpekris, F. Stylianopoulos, T. (2022) “Nanomechanical signature of fibrosarcoma: from single cells to tissue level”, ESB2022, 27^th Congress of the European Society of Biomechanics, 26-26 June, Porto, Portugal (podium)
Stylianou, A., Kontomaris, S.V., Malamou, A., (2022) “Determining tip radius in AFM nanoindentation”, ESB2022, 27^th Congress of the European Society of Biomechanics, 26-26 June, Porto, Portugal (poster)
Stylianou, A., Voutouri, C., Mpekris, F., Papouis,A., Polemidiotou, K., Stylianopoulos T., (2022) “Solid tumors nanomechanical fingerprints and collagen-based optical signatures”, Tissue Optics and Photonics II, SPIE Photonics Europe, 3 – 7 April, Strasbourg, France (podium)
Stylianou, A., ” Atomic force microscopy based nanomechanical biomarkers” 9th International Multithematic Bio-Medical Congress (IMBMC), 18 – 20 November, Nicosia, Cyprus (poster)
Stylianou, A. (2021) “Nanomechanical and Optical Collagen-based Signatures as Cancer Biomarkers” 7th Freiberg Collagen Symposium September 29 and 30, Germany (online format, invited, podium)
Stylianou, A. (2021) “Nanomechanical assessment of cancer cells and solid tumors as a mechanical biomarker” ESCHM-ISCH-ISB, symposium Nanomechanical and nanorheological assessments of various diseases, July 4-7, Fukuoka, Japan, (online format, invited, podium)
Stylianou, A., Mpekris, F., Voutouri, C., Stylianopoulos T., (2021) “Nanomechanical finger-prints of solid tumors as a treatment monitoring biomarker”, 26th Congress of the European Society of Biomechanics, Jully 11-14, Milan, Italy (online format, podium)
Stylianou, A., Voutouri, C., Mpekris, F., Stylianopoulos T., (2021) “Nanomechanical Signature of sarcomas can be predictive of response to chemotherapy”, SB3C2021 Summer Biomechanics, Bioengineering and Biotransport Conference, June 14-18, USA (virtual, podium)
Stylianou, A., Voutouri, C., Mpekris, F., Stylianopoulos T., (2021) “Pancreatic Cancer Collagen-Based Optical Signatures”, Polarized light and Optical Angular Momentum for biomedical diagnostics, SPIE BiOS, March 6 – 11, San Francisco, California, USA (online format, podium).
Stylianou, A. (2019) “Extracellular matrix stiffness and transforming growth factor-ß modulates pancreatic stromal cells’ cytoskeletal remodeling” 10th Annual Symposium ”Physics of Cancer”, 25-27, Leipzig, Germany (podium).
Stylianou, A. (2018) “Cell and tissue nanomechanical properties in cancer research” 2nd “Current Advances in Cancer Research” Colloquium, 13 December 2018, European University, Nicosia, Cyprus (podium).
Stylianou, A, Gkretsi, V. and Stylianopoulos, T. (2018) “Pancreatic Cancer Associated Fibroblasts Stiffness and Invasion Properties are Modulated by Transforming Growth Factor-β” 8th World Congress of Biomechanics, 8-12 July, Dublin, Ireland (podium).
Stylianou, A, Gkretsi, V. and Stylianopoulos, T. (2017) “Normal and Cancer Associated Pancreatic Fibroblasts Shape Modulation is mediated by Transforming Growth Factor β and Matrix Stiffness”, AFM Biomed Conference, 4-8 September 2017, Krakow, Poland (podium).
Stylianou, A, Stylianopoulos, T. (2016) “Investigation of the effect of Tumor Growth Factor-β on Pancreatic Normal and Cancer Associated Fibroblasts”, Cyprus Researchers’ Night 2016, 30 September, Nicosia, Cyprus (poster).
Stylianou, A, Gkretsi, V. and Stylianopoulos, T. (2016) “Effects of Tumor Growth Factor β on Pancreatic Cancer Associated Fibroblasts and Fibroblasts”, EUROAFMForum 2016, June, 22-24, 2016, Geneva, Switzerland (podium).
Stylianou, A., Kontomaris, S.V., Yova, D (2016) “Probing Collagen Nanocharacteristics After Low-Level Red Laser Irradiation” XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016, March 31st–April 2nd 2016, Paphos, Cyprus (podium).
Kontomaris, S.V., Yova, D, Sambani, k and Stylianou, A. (2016) ” AFM Investigation of the Influence of Red Light Irradiation on Collagen” XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016, March 31st–April 2nd 2016, Paphos, Cyprus (podium).
Stylianou, A., Yova, D., (2014) “Optical Radiation Effects on Collagen Topology Studied with AFM and SEM”, Heracleitus II Dissemination Workshop, 22 May 2014, NTUA, Athens, Greece, (poster).
Stylianou, A., Yova, D., Alexandratou, E. (2013) “Investigation of UV interactions with collagen thin films by using AFM microscopy imaging”, 8^th Meeting of Hellenic Society for Biomaterials, 15-16 November 2013, Athens, Greece (podium).
Stylianou, A., Yova, D., Alexandratou, E. (2013) “Assessing Fibroblasts Response on Collagen Thin Films Nanotopography by Atomic Force Microcopy”, ELEVIT 2013, 5^th Panhellenic Conference on Biomedical Technologies, 4-6 April 2013, Athens, Greece (poster).
Stylianou, A., Yova, D., Alexandratou, Ε., Petri, Α. (2013) “Atomic force imaging microscopy investigation of the interaction of ultraviolet radiation with collagen thin films”, SPIE Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 85940E, part of BIOS 2013, 2-7 February 2013, San Francisco, California, USA (podium).
Stylianou, A., Yova, D., Politopoulos, K. (2012) “Atomic Force Microscopy Surface Nanocharacterization of UV-Irradiated Collagen Thin Films”, IEEE 12th International Conference on Bioinformatics & Bioengineering, BIBE 2012, 11-13 November 2012, Larnaca, Cyprus (podium).
Kontomaris, S.V., Stylianou, A., Yova, D., Politopoulos, K (2012) “Mechanical Properties of Collagen Fibrils on Thin Films by Atomic Force Microscopy Nanoindentation”, 12^th International Conference on Bioinformatics & Bioengineering, BIBΕ 2012, 11-13 November, 2012, Larnaca, Cyprus (podium).
Stylianou, A., Kontomaris, S.V. and Yova, D. (2012) “Nanocharacterization of UV-Irradiated Collagen Thin Films by Atomic Force Microscopy Imaging and Nanoindentation”, Fibrous Protein Nanocomposities for Tailored Hybrid Biostructures and Devices-ECI, 8-12 October, 2012, Daios Cove Resort, Crete, Greece (poster).
Stylianou, A., Yova, D., Petri, A. (2012) “AFM Characterization of Collagen Thin Films for Assessing Fibroblasts Response on Collagen Nanotopography”, 9^th International Conference on Nanoscience & Nanotechnologies, 3-6 July, 2012, Thessaloniki, Greece (poster).
Stylianou, A., Yova, D., Politopoulos, K. (2012) “Development and Atomic Force Microscopy Nanoscale Characterization of Collagen Thin Films for Medical and Biological Applications”, 4^th Panhellenic Conference on Biomedical Technologies, International Federation for Medical and Biological Engineering, ELEVIT 2012, 20-21 January 2012, NTUA, Athens, Greece (poster).
Stylianou, A., Yova, D., Politopoulos, K. (2011) “Collagen Thin Films Development for Biomedical Applications”, 6^st Meeting of Hellenic Society for Biomaterials, November 18-19, Athens, Greece (podium).
Stylianou, A., Yova, D., Politopoulos, K. (2012) “Atomic Force Microscopy Quantitative and Qualitative Nanoscale Characterization of Collagen Thin Films”, Emerging Technologies in Non-Destructive Testing V- 5^th Conference on Emerging Technologies in NDT, 19-21 September 2011, Ioannina, Greece (podium).
Stylianou, A., Politopoulos, K., Yova, D. (2011) “Atomic Force Microscopy Imaging of the Nanoscale Assembly of Type I Collagen on Controlled Polystyrene Particles Surfaces”, 5^th European IFMBE Conference, EMBEC 2011, 14-18 September 2011, Budapest, Hungary (poster).
Stylianou, A., Kontomaris, S.B., Kyriazi, M., Yova D. (2010) “Surface characterization of collagen films by atomic force microscopy”, 12^th Mediterranean Conference on Medical and Biological Engineering and Computing, MEDICON 2010, 27-30 May 2010, Chalkidiki, Greece (podium).
Stylianou, A., Kyriazi, M., Politopoulos, K., Yova, D. (2009) “Combined SHG signal information with AFM imaging to assess conformational changes in collagen”, 9^th International Conference on Information Technology and Applications in Biomedicine, IEEE ITAB 2009, 4-7 November 2009, Larnaca, Cyprus (poster).
Stylianou, A., Kyriazi, M., Politopoulos, K., Yova, D. (2009) “Second harmonic generation and atomic force microscopy study of collagen thin films” 4^th International Graduate Summer School: Biophotonics ′09, 6-13 June 2009, Ven, Sweden (poster).
Stylianou, A., Kyriazi, M., Yova, D. (2009) “Study of the influence of physicochemical factors in the creation of thin collagen films with atomic force microscopy”, 1^st Meeting Hellenic Society for Biomaterials & H.A.O.S.T, 27-29 November 2009, Athens, Greece (podium).
Stylianou, A., Kyriazi, M., Yova, D. (2009) “Study of collagen type I with AFM”, 3^rd Meeting of Hellenic Society for Biomaterials, 21-22 November 2008, Athens, Greece (poster).

Conference Presentations (presented by other)

Kontomaris, S.V., Stylianou, A (2025) “Overcoming challenges in the AFM mechanical nanocharacterization of soft heterogeneous biological materials”, AFM BioMed Conference 2025, 8^th-11^th April 2025, Barcelona, Spain (podium)
Polemidiotou, K., Stylianou, A., (2022) “Collagen content and stiffness alter the cytoskeleton and nanomechanical properties of sarcoma cells”, 1st Βιος-SBSCy Conference, 22-23 October, Nicosia, Cyprus (poster)
Polemidiotou, K., Stylianou, A., (2022) “Collagen content and stiffness alter the cytoskeleton and nanomechanical properties of sarcoma cells”, 2022 NaoScientific Forum Europe, 6-7 October, (online, podium)
Polemidiotou, K., Stylianou, A., (2021) “Collagen content and stiffness cause cytoskeletal remodelling in sarcoma cells” 9th International Multithematic Bio-Medical Congress (IMBMC), 18 – 20 November, Nicosia, Cyprus (podium)
Stavrou, M., Papaparaskeva, G., Stathis, A., Stylianou, A., Turcu, R., Krasia-Christoforou, T. and Couri, S. (2021) “Synthesis, Characterization and Nonlinear Optical Response of Polyelectrolyte-Stabilised Copper Hydroxide and Copper Oxide Colloidal Nanohybrids”, XXXV Panhellenic Conference on Solid State Physics and Material Science, 26-29 September 2021, Athens, Greece (virtual, poster).
Kontomaris, S.V., Malamou, A., Stylianou, A. (2021) “Indentation method using spherical indenters: Determing the errors while using Hertz equation”, 26th Congress of the European Society of Biomechanics, Jully 11-14, Milan, Italy (online format, poster)
Kapnisis, K., Stylianou, A., Kokkinidou, D., Prokopi, M., Anayiotos, A. (2021) “Lymphocyte nanomechanics in response to strent implantation”, SB3C2021 Summer Biomechanics, Bioengineering and Biotransport Conference, June 14-18, USA (virtual, podium)
Louca, M., Stylianou A., Minia, A., Pliaka, V., Alexopoulos, L., Gkretsi, V., and Stylianopoulos, T. (2018) “Opposing effects of RSU-1 silencing in glioma cell invasion depending on their aggressiveness” 6^th International Multithematic Bio-Medical Congress (IMBMC), Bio-Medical Scientific Cyprus (BSC), 15-17 November, 2018, Nicosia, Cyprus (1^st poster award) (poster).
Louca, M., Stylianou, A., Gkretsi, V., and Stylianopoulos, T. (2018), “The Role of RSU-1 in Glioma Cells”, 25th Biennial Congress of the European Association for Cancer Research (EACR25) 30 June – 03 July, Amsterdam (poster).
Gkretsi V., Louca M., Stylianou A., Minadakis G., Spyrou G., and Stylianopoulos T. (2018) “Inhibition of breast cancer cell invasion by Ras suppressor-1 (RSU-1) silencing is reversed by Growth Differentiation Factor-15 (GDF-15)”, 7th International Conference of the Cyprus Society of Human Genetics, December 7th 2018, Nicosia, Cyprus (podium).
Gkretsi V., Stylianou A., Louca M., and Stylianopoulos T. (2017) “Identification of Ras Suppressor-1 (RSU-1) and Vasodilator-Stimulated Phosphoprotein (VASP) as potential breast cancer metastasis biomarkers using a three-dimensional in vitro approach”, 7th International Conference on Tumor-Host Interaction and Angiogenesis, 25-28 June, 2017, Monte Verità, Ascona, Switzerland (poster).
Gkretsi, V., Stylianou, A, Stylianopoulos, T. (2016) “Targeting breast cancer metastasis: A bioengineering approach”, 21st World Congress on Advances in Oncology & 19th International Symposium of Molecular Medicine, 6-8 October, 2016, Athens, Greece (podium).
Stylianou, A., Kontomaris, S.V., Yova, D., Balogiannis, G. (2013) “AFM Multimode Imaging and Nanoindetation Method For Assessing Collagen Nanoscale Thin Films Heterogeneity” XIII Mediterranean Conference on Medical and Biological Engineering and Computing – MEDICON 2013, 25-28 September 2013, Sevilla, Spain (podium).
Kontomaris, S. V., Stylianou, Α., Yova, D. (2013) “Investigation of collagen thin films mechanical heterogeneity in nanoscale using AFM”, 8^th Meeting of Hellenic Society for Biomaterials, 15- 16 November 2013, Athens, Greece (podium).
Kontomaris, S.V., Yova, D., Stylianou, A., Sampani, K., Balogiannis, G., Politopoulos, K. (2013) “Mechanical Heterogeneity of Collagen Fibrils”, 5^th Panhellenic Conference on Biomedical Technologies, ELEVIT 2013, 4-6 April 2013, Athens, Greece (poster).