Publication List
Journal Paper Publications (Peer-reviewed)
Mathematical modeling of tumor nanomechanical fingerprints: A weighted skew-normal distribution approach for cancer diagnosis and treatment monitoring
SV Kontomaris, I Psychogios, A Malamou, T Stylianopoulos, A Stylianou
Next Nanotechnology 9, 100365, 2026
Pharmacological Targeting of Midkine (MDK) Reveals Stiffness-Dependent Control of Hepatocellular Carcinoma Invasiveness
C Christou, K Agathangelou, N Dietis, A Stylianou, V Gkretsi
International Journal of Molecular Sciences 27 (4), 1766, 2026
A Practical Approach for Determining Depth-Dependent Mechanical Properties of Soft Materials in AFM Indentation via Polynomial Fitting and a New Model for Cellular Mechanics
SV Kontomaris, A Malamou, I Psychogios, A Stylianou
Eng 7 (2), 75, 2026
Mechanical Nonlinear Oscillations Using a Hertzian-Type Restoring Force
SV Kontomaris, GM Ismail, A Malamou, A Stylianou
Vibration 8 (4), 74, 2025
Atomic Force Microscopy‐Based Nanomechanical Signatures for Staging Classification and Drug Response in Pulmonary Fibrosis
A Stylianou, K Polemidiotou, V Alimisis, F Mpekris, C Voutouri, M Mari, ...
Small 21 (44), e04526, 2025
Beyond Hertz: Accurate Analytical Force–Indentation Equations for AFM Nanoindentation with Spherical Tips
SV Kontomaris, A Malamou, GM Ismail, A Katsiki, A Stylianou
Metrology 5 (4), 63, 2025
Global linear modeling of AFM indentation curves for soft samples with various indenter geometries
SV Kontomaris, A Malamou, GM Ismail, I Psychogios, A Stylianou
Next Materials 9, 101299, 2025
Quantitative criteria for the validity of the elastic half-space assumption in AFM nanoindentation
SV Kontomaris, I Psychogios, M Stergiopoulos, A Malamou, A Stylianou
Next Materials 9, 101180, 2025
Photodynamic Therapy and Tumor Microenvironment-Targeting Strategies: A Novel Synergy at the Frontier of Cancer Treatment
S Torna, V Gkretsi, A Stylianou
International Journal of Molecular Sciences 26 (17), 8588, 2025
The Young’s modulus as a mechanical biomarker in AFM experiments: A tool for cancer diagnosis and treatment monitoring
SV Kontomaris, A Malamou, A Stylianou
Sensors 25 (11), 3510, 2025
A Novel Approach to Calculate the Range of High-Energy Charged Particles Within a Medium
I Psychogios, SV Kontomaris, A Malamou, A Stylianou
Atoms 13 (5), 38, 2025
Monitoring the Distance and Velocity of Protons in a Medium for Biomedical Applications Using a Straightforward Mathematical Approach
SV Kontomaris, I Psychogios, A Malamou, A Stylianou
Sci 7 (2), 49, 2025
In vitro models: Can they unravel the complexities of cancer cell metastasis?
S Pafitanis, LC Zacharia, A Stylianou, V Gkretsi
Biochimica et Biophysica Acta (BBA)-Reviews on Cancer 1880 (2), 189293, 2025
Interdependent roles for growth differentiation factor-15 (GDF15) and LIMS1 in regulating cell migration: Implications for colorectal cancer metastasis
A Kotsoni, LV Kozaki, A Stylianou, V Gkretsi
Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1872 (3), 119904, 2025
Development of an accurate simplified approach for data processing in AFM indentation experiments
SV Kontomaris, A Malamou, A Stylianou
Micron 190, 103782, 2025
Simplifying Data Processing in AFM Nanoindentation Experiments on Thin Samples
SV Kontomaris, A Malamou, A Stylianou
Eng 6 (2), 32, 2025
Open‐Source Tools for Assessing Cytoskeleton Properties in Pathological Conditions From Microscopy Images: An Application Note
M Petrou, A Stylianou
Applied Research 4 (1), e70005, 2025
Atomic force microscopy reveals the influence of substrate collagen concentration and TGF-β on lung fibroblast mechanics
AC Karagkouni, K Polemidiotou, V Gkretsi, A Stylianou
Micron 189, 103751, 2025
Utilizing collagen-coated hydrogels with defined stiffness as calibration standards for AFM experiments on soft biological materials: the case of lung cells and tissue
A Stylianou, SV Kontomaris, K Polemidiotou, A Zachariades
Methods in Microscopy 1 (2), 151-162, 2025
Assessing sarcoma cell cytoskeleton remodeling in response to varying collagen concentration
K Polemidiotou, SG Kulkarni, R Szydlak, M Lekka, M Radmacher, ...
International Journal of Biological Macromolecules 282, 136770, 2024
Fluorescence Microscopy of the Cell and Cell Interactions to Study Dynamic Cellular Processes
A Stylianou, V Gkretsi, V Huntošová, A Theodosiou
Bioimaging in Tissue Engineering and Regeneration: Advanced Microscopy and …, 2024
Accurate Modelling of AFM Force-Indentation Curves with Blunted Indenters at Small Indentation Depths
SV Kontomaris, A Malamou, A Stylianou
Micromachines 15 (10), 1209, 2024
Towards simpler modelling expressions for the mechanical characterization of soft materials
SV Kontomaris, A Malamou, A Stylianou, G Chliveros
Micro and Nanosystems 16 (3), 172-182, 2024
Adaptive fiber optic fluorescence analysis for cancer tissues via collagen detection
A Theodosiou, A Stylianou
Biomedical Spectroscopy, Microscopy, and Imaging III 13006, 226-231, 2024
Stabilizing tumor-resident mast cells restores T-cell infiltration and sensitizes sarcomas to PD-L1 inhibition
M Panagi, F Mpekris, C Voutouri, AG Hadjigeorgiou, C Symeonidou, ...
Clinical Cancer Research 30 (11), 2582-2597, 2024
A Linear Fit for Atomic Force Microscopy Nanoindentation Experiments on Soft Samples
SV Kontomaris, A Malamou, A Zachariades, A Stylianou
Processes 12 (4), 843, 2024
Short-Term l-arginine Treatment Mitigates Early Damage of Dermal Collagen Induced by Diabetes
I Miler, MD Rabasovic, S Askrabic, A Stylianou, B Korac, A Korac
Bioengineering 11 (4), 407, 2024
Advances in optical fiber speckle sensing: a comprehensive review
I Chapalo, A Stylianou, P Mégret, A Theodosiou
Photonics 11 (4), 299, 2024
Cancer treatment using bosentan in combination with a checkpoint inhibitor
JD Martin, T Stylianopoulos, F MPEKRIS, M PANAGI, C VOUTOURI, ...
US Patent App. 18/256,776, 2024
Midkine (MDK) in hepatocellular carcinoma: more than a biomarker
C Christou, A Stylianou, V Gkretsi
Cells 13 (2), 136, 2024
A new method for AFM mechanical characterization of heterogeneous samples with finite thickness
SV Kontomaris, A Malamou, A Stylianou
Journal of Mechanics 40, 552-564, 2024
Overcoming challenges and limitations regarding the atomic force microscopy imaging and mechanical characterization of nanofibers
SV Kontomaris, A Stylianou, G Chliveros, A Malamou
Fibers 11 (10), 83, 2023
A new elementary method for determining the tip radius and Young’s modulus in AFM spherical indentations
SV Kontomaris, A Stylianou, G Chliveros, A Malamou
Micromachines 14 (9), 1716, 2023
Multilevel assessment of stent-induced inflammation in the adjacent vascular tissue
K Kapnisis, A Stylianou, D Kokkinidou, A Martin, D Wang, PG Anderson, ...
ACS Biomaterials Science & Engineering 9 (8), 4747-4760, 2023
Fascin-1 in cancer cell metastasis: old target-new insights
E Sarantelli, A Mourkakis, LC Zacharia, A Stylianou, V Gkretsi
International journal of molecular sciences 24 (14), 11253, 2023
Pancreatic cancer presents distinct nanomechanical properties during progression
A Stylianou, C Voutouri, F Mpekris, T Stylianopoulos
Annals of Biomedical Engineering 51 (7), 1602-1615, 2023
AFM indentation on highly heterogeneous materials using different indenter geometries
SV Kontomaris, A Stylianou, G Chliveros, A Malamou
Applied Mechanics 4 (2), 460-475, 2023
3D AFM nanomechanical characterization of biological materials
SV Kontomaris, A Stylianou, A Georgakopoulos, A Malamou
Nanomaterials 13 (3), 395, 2023
Determining spatial variability of elastic properties for biological samples using AFM
SV Kontomaris, A Stylianou, G Chliveros, A Malamou
Micromachines 14 (1), 182, 2023
AFM Indentation on Highly Heterogeneous Materials Using Different Indenter Geometries. Appl. Mech. 2023, 4, 460–475
SV Kontomaris, A Stylianou, G Chliveros, A Malamou
3D AFM Nanomechanical Characterization of Biological Materials. Nanomaterials 2023, 13, 395
SV Kontomaris, A Stylianou, A Georgakopoulos, A Malamou
A New Elementary Method for Determining the Tip Radius and Young’s Modulus in AFM Spherical Indentations. Micromachines 2023, 14, 1716
SV Kontomaris, A Stylianou, G Chliveros, A Malamou
Is it mathematically correct to fit AFM data (obtained on biological materials) to equations arising from Hertzian mechanics?
SV Kontomaris, A Stylianou, A Georgakopoulos, A Malamou
Micron 164, 103384, 2023
Nanomechanical properties of solid tumors as treatment monitoring biomarkers
A Stylianou, F Mpekris, C Voutouri, A Papoui, A Constantinidou, E Kitiris, ...
Acta Biomaterialia 154, 324-334, 2022
Polymeric micelles effectively reprogram the tumor microenvironment to potentiate nano-immunotherapy in mouse breast cancer models
M Panagi, F Mpekris, P Chen, C Voutouri, Y Nakagawa, JD Martin, T Hiroi, ...
Nature Communications 13 (1), 7165, 2022
Solid tumors nanomechanical fingerprints and collagen-based optical signatures
A Stylianou, C Voutouri, F Mpekris, A Papoui, K Polemidiotou, ...
Tissue Optics and Photonics II 12147, 108-114, 2022
The Hertzian theory in AFM nanoindentation experiments regarding biological samples: Overcoming limitations in data processing
SV Kontomaris, A Malamou, A Stylianou
Micron 155, 103228, 2022
Atomic force microscopy nanoindentation method on collagen fibrils
SV Kontomaris, A Stylianou, A Malamou
Materials 15 (7), 2477, 2022
Assessing collagen D-band periodicity with atomic force microscopy
A Stylianou
Materials 15 (4), 1608, 2022
Research Article Is It Possible to Directly Determine the Radius of a Spherical Indenter Using Force Indentation Data on Soft Samples?
SV Kontomaris, A Stylianou, A Malamou
À Á 100, R2, 2022
Is It Possible to Directly Determine the Radius of a Spherical Indenter Using Force Indentation Data on Soft Samples?
SV Kontomaris, A Stylianou, A Malamou
Scanning 2022 (1), 6463063, 2022
Synthesis, characterization and nonlinear optical response of polyelectrolyte-stabilized copper hydroxide and copper oxide colloidal nanohybrids
M Stavrou, G Papaparaskeva, A Stathis, A Stylianou, R Turcu, ...
Optical Materials 119, 111329, 2021
Endothelin inhibition potentiates cancer immunotherapy revealing mechanical biomarkers predictive of response
C Voutouri, M Panagi, F Mpekris, A Stylianou, C Michael, MA Averkiou, ...
Advanced Therapeutics 4 (9), 2000289, 2021
The average Young's modulus as a physical quantity for describing the depth-dependent mechanical properties of cells
SV Kontomaris, A Georgakopoulos, A Malamou, A Stylianou
Mechanics of Materials 158, 103846, 2021
Lymphocyte Nanomechanics in Response to Stent Implantation
K Kapnisis, A Stylianou, D Kokkinidou, M Prokopi, A Anayiotos
Summer Biomechanics, Bioengineering, and Biotransport Conference, 2021
Atomic force microscopy and spectroscopy
M Lekka, A Stylianou, B Plochberger
Imaging Modalities for Biological and Preclinical Research: A Compendium …, 2021
Transmission light microscopy
A Stylianou, V Gkretsi, P Sampaio, M Glösmann, A Walter
Imaging Modalities for Biological and Preclinical Research: A Compendium …, 2021
Correlative atomic force microscopy
A Miranda, AI Gómez-Varela, CC Moura, PAA De Beule, M Sarna, ...
Imaging Modalities for Biological and Preclinical Research: A Compendium …, 2021
Pancreatic cancer collagen-based optical signatures
A Stylianou, C Voutouri, F Mpekris, T Stylianopoulos
Polarized Light and Optical Angular Momentum for Biomedical Diagnostics …, 2021
A discussion regarding the application of the Hertz contact theory on biological samples in AFM nanoindentation experiments
SV Kontomaris, A Stylianou, KS Nikita, A Malamou
Micro and Nanosystems 13 (1), 42-48, 2021
Is it Necessary to Calculate Young’s Modulus in AFM Nanoindentation Experiments Regarding Biological Samples?
SV Kontomaris, A Malamou, A Stylianou
Micro and Nanosystems 13 (1), 3-8, 2021
rsc. li/nanoscale
C Huang, G Chen, A Nashalian, J Chen, A Miranda, AI Gómez-Varela, ...
Nanoscale 13 (2065), 2338-2349, 2021
Nanomechanical assessment of cancer cells and solid tumors as a mechanical biomarker
A Stylianou
BIORHEOLOGY 58 (3-4), 151-151, 2021
How did correlative atomic force microscopy and super-resolution microscopy evolve in the quest for unravelling enigmas in biology?
A Miranda, AI Gómez-Varela, A Stylianou, LM Hirvonen, H Sánchez, ...
Nanoscale 13 (4), 2082-2099, 2021
Silencing of growth differentiation factor-15 promotes breast cancer cell invasion by down-regulating focal adhesion genes
V Gkretsi, A Stylianou, M Kalli, M Louca, C Voutouri, A Zaravinos, ...
Anticancer Research 40 (3), 1375-1385, 2020
TGF-β inhibition combined with cytotoxic nanomedicine normalizes triple negative breast cancer microenvironment towards anti-tumor immunity
M Panagi, C Voutouri, F Mpekris, P Papageorgis, MR Martin, JD Martin, ...
Theranostics 10 (4), 1910, 2020
A New Approach for the AFM‐Based Mechanical Characterization of Biological Samples
SV Kontomaris, A Malamou, A Stylianou
Scanning 2020 (1), 2896792, 2020
Determination of the linear elastic regime in AFM nanoindentation experiments on cells
SV Kontomaris, A Stylianou, KS Nikita, A Malamou
Materials Research Express 6 (11), 115410, 2019
A simplified approach for the determination of fitting constants in Oliver–Pharr method regarding biological samples
SV Kontomaris, A Stylianou, KS Nikita, A Malamou, T Stylianopoulos
Physical Biology 16 (5), 056003, 2019
Ras suppressor-1 (RSU-1) promotes cell invasion in aggressive glioma cells and inhibits it in non-aggressive cells through STAT6 phospho-regulation
M Louca, A Stylianou, A Minia, V Pliaka, LG Alexopoulos, V Gkretsi, ...
Scientific reports 9 (1), 7782, 2019
Atomic Force Microscopy: In Sickness and in Health
SV Kontomaris, C Grant, E Alexandratou, A Stylianou
Scanning 2019, 6149247, 2019
Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts
A Stylianou, V Gkretsi, M Louca, LC Zacharia, T Stylianopoulos
Journal of the Royal Society Interface 16 (154), 2019
An alternative approach for the Young’s modulus determination of biological samples regarding AFM indentation experiments
SV Kontomaris, A Stylianou, A Malamou, KS Nikita
Materials Research Express 6 (2), 025407, 2019
Inhibition of breast cancer cell invasion by Ras suppressor-1 (RSU-1) silencing is reversed by growth differentiation factor-15 (GDF-15)
V Gkretsi, M Louca, A Stylianou, G Minadakis, GM Spyrou, ...
International journal of molecular sciences 20 (1), 163, 2019
Atomic force microscopy on biological materials related to pathological conditions
A Stylianou, SV Kontomaris, C Grant, E Alexandratou
Scanning 2019 (1), 8452851, 2019
A discussion regarding the approximation of cylindrical and spherical shaped samples as half spaces in AFM nanoindentation experiments
SV Kontomaris, A Stylianou, A Malamou, T Stylianopoulos
Materials Research Express 5 (8), 085402, 2018
Transforming growth factor-β modulates pancreatic cancer associated fibroblasts cell shape, stiffness and invasion
A Stylianou, V Gkretsi, T Stylianopoulos
Biochimica et Biophysica Acta (BBA)-General Subjects 1862 (7), 1537-1546, 2018
AFM assessing of nanomechanical fingerprints for cancer early diagnosis and classification: from single cell to tissue level
A Stylianou, M Lekka, T Stylianopoulos
Nanoscale 10 (45), 20930-20945, 2018
Atomic force microscopy nano-characterization of 3D collagen gels with tunable stiffness
A Stylianou, V Gkretsi, T Stylianopoulos
MethodsX 5, 503-513, 2018
Exploring the nano-surface of collagenous and other fibrotic tissues with AFM
A Stylianou, V Gkretsi, CS Patrickios, T Stylianopoulos
Fibrosis: Methods and Protocols, 453-489, 2017
Atomic force microscopy for university students: applications in biomaterials
SV Kontomaris, A Stylianou
European Journal of Physics 38 (3), 033003, 2017
Vasodilator-Stimulated Phosphoprotein (VASP) depletion from breast cancer MDA-MB-231 cells inhibits tumor spheroid invasion through downregulation of Migfilin, β-catenin and …
V Gkretsi, A Stylianou, T Stylianopoulos
Experimental Cell Research 352 (2), 281-292, 2017
Identification of Ras suppressor-1 (RSU-1) as a potential breast cancer metastasis biomarker using a three-dimensional in vitro approach
V Gkretsi, A Stylianou, M Louca, T Stylianopoulos
Oncotarget 8 (16), 27364, 2017
Big data in healthcare: a discussion on the big challenges
ASMA Talias
Health and Technology 7 (1), 97-107, 2017
Atomic force microscopy for collagen‐based nanobiomaterials
A Stylianou
Journal of Nanomaterials 2017 (1), 9234627, 2017
Investigation of the mechanical properties of collagen fibrils under the influence of low power red laser irradiation
SV Kontomaris, A Stylianou, D Yova
Biomedical Physics & Engineering Express 2 (6), 064002, 2016
AFM investigation of the influence of red light irradiation on collagen
SV Kontomaris, D Yova, K Sambani, A Stylianou
XIV Mediterranean Conference on Medical and Biological Engineering and …, 2016
Probing collagen nanocharacteristics after low-level red laser irradiation
A Stylianou, SV Kontomaris, D Yova
XIV Mediterranean Conference on Medical and Biological Engineering and …, 2016
Atomic force microscopy probing of cancer cells and tumor microenvironment components
A Stylianou, T Stylianopoulos
BioNanoScience 6 (1), 33-46, 2016
Targeting breast cancer metastasis: A bioengineering approach
V Gkretsi, A Stylianou, T Stylianopoulos
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 38, S37-S37, 2016
The significance of the percentage differences of Young’s modulus in the AFM nanoindentation procedure
SV Kontomaris, D Yova, A Stylianou, K Politopoulos
Micro and Nanosystems 7 (2), 86-97, 2015
Atomic force microscopy investigation of the interaction of low-level laser irradiation of collagen thin films in correlation with fibroblast response
A Stylianou, D Yova
Lasers in medical science 30 (9), 2369-2379, 2015
Remodeling components of the tumor microenvironment to enhance cancer therapy
V Gkretsi, A Stylianou, P Papageorgis, C Polydorou, T Stylianopoulos
Frontiers in oncology 5, 214, 2015
The ‘magic light’: A discussion on laser ethics
A Stylianou, MA Talias
Science and engineering ethics 21 (4), 979-998, 2015
The effects of UV irradiation on collagen D‐band revealed by atomic force microscopy
SV Kontomaris, D Yova, A Stylianou, G Balogiannis
Scanning 37 (2), 101-111, 2015
Investigation of the influence of UV irradiation on collagen thin films by AFM imaging
A Stylianou, D Yova, E Alexandratou
Materials Science and Engineering: C 45, 455-468, 2014
Assessing collagen nanoscale thin films heterogeneity by AFM multimode imaging and nanoindetation for nanobiomedical applications
A Stylianou, S VasileiosKontomaris, D Yova
Micro and Nanosystems 6 (2), 95-102, 2014
Επίδραση οπτικής ακτινοβολίας στην τοπολογία κολλαγόνου με χρήση απεικονιστικής μικροσκοπίας ατομικής δύναμης και σάρωσης ηλεκτρονίων
Α Στυλιανού
Εθνικό Μετσόβιο Πολυτεχνείο (ΕΜΠ). Σχολή Ηλεκτρολόγων Μηχανικών και …, 2014
AFM multimode imaging and nanoindetation method for assessing collagen nanoscale thin films heterogeneity
A Stylianou, SV Kontomaris, D Yova, G Balogiannis
XIII Mediterranean Conference on Medical and Biological Engineering and …, 2014
Surface nanoscale imaging of collagen thin films by Atomic Force Microscopy
A Stylianou, D Yova
Materials Science and Engineering: C 33 (5), 2947-2957, 2013
Book chapters
- Stylianou, A. Gkretsi, V., Huntosova, V., Theodosiou, A. (2024) “Fluorescence Microscopy of the Cell & Cell Interactions to Study Dynamic Cellular Processes”, In: Walter, A., Slezak, P., Müller, R., Kerckhofs, G. and Bajoghli, B. (eds) Tissue Engineering and Regeneration. Bioimaging in Tissue Engineering and Regeneration, Springer-Nature, https://doi.org/10.1007/978-3-030-85569-7_1-1
- Stylianou, A., Gkretsi, V., Walter, A. , Glösmann, M. (2021) “Part I: Ex-vivo Imaging, I.1 – Light and fluorescence microscopy”, In: Walter, A., Mannheim, J., Caruana, C.J. (eds) Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 1, IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology, Hardback ISBN: 9780750330572, Ebook ISBN: 9780750330596 , https://doi.org/10.1088/978-0-7503-3059-6ch1
- Miranda, A., Dykas, M., Gómez, A., De Beule, P., Moura, K. and Stylianou, A., (2020) ” Part III: Correlative multimodal imaging and image data fusion, III.1.b Correlative atomic force microscopy”, In: Walter, A., Mannheim, J., Caruana, C.J. (eds) Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 2, IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology, Hardback ISBN: 9780750337458, Ebook ISBN: 9780750337472. https://doi.org/10.1088/978-0-7503-3747-2ch17
- Lekka, M., Stylianou, A. and Plochberger, B. (2020) “Part I: Ex-vivo Imaging, I.6 – Atomic force microscopy and spectroscopy”, In: Walter, A., Mannheim, J., Caruana, C.J. (eds) Imaging Modalities for Biological and Preclinical Research: A Compendium, Volume 1, IOP-IPEM ebook Series in Physics and Engineering in Medicine and Biology, Hardback ISBN: 9780750330572, Ebook ISBN: 9780750330596, https://doi.org/10.1088/978-0-7503-3059-6ch30
- Stylianou, A., Gkretsi, V., Patrickios, C.S., Stylianopoulos, T. (2017) “Exploring the nano-surface of collagenous and other fibrotic tissues with AFM” In: Rittié L. (eds) Fibrosis. Methods in Molecular Biology, vol 1627, pp. 453-489, Humana Press, New York, NY, doi: 0.1007/978-1-4939-7113-8_29
Research Publications in Referred Conference Proceedings
- Theodosiou, A. and Stylianou, A. (2024) “Adaptive fiber optic fluorescence analysis for cancer tissues via collagen detection”, Proceedings of SPIE, Biomedical Spectroscopy, Microscopy, and Imaging III, 13006, 226-231, https://doi.org/10.1117/12.3014718
- Stylianou, A., Voutouri, C., Mpekris, F., Papoui, A., Polemidiotou, K., Stylianopoulos T. (2022) “Solid tumors nanomechanical fingerprints and collagen-based optical signatures”, SPIE, Proceedings Volume 121470I, Tissue Optics and Photonics II, https://doi.org/10.1117/12.2621002
- Stylianou, A., Voutouri, C., Mpekris, F., Stylianopoulos T., (2021) “Pancreatic Cancer Collagen-Based Optical Signatures”, Proceedings of SPIE Volume 11646, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics; 1164613, org/10.1117/12.2582590.
- Gkretsi, V., Stylianou, A, Stylianopoulos, T. (2016) “Targeting breast cancer metastasis: A bioengineering approach”, International Journal of Molecular Medicine, 38, S1. pp S37.
- Stylianou, A., Kontomaris, S.V., Yova, D (2016) “Probing Collagen Nanocharacteristics After Low-Level Red Laser Irradiation” IFMBE Proceedings, 57, 264-268, doi: 10.1007/978-3-319-32703-7_53
- Kontomaris, S.V., Yova, D, Sambani, k and Stylianou, A. (2016) ” AFM Investigation of the Influence of Red Light Irradiation on Collagen” IFMBE Proceedings, 57, 269-274, doi:10.1007/978-3-319-32703-7_54
- Stylianou, A., Kontomaris, S.V., Yova, D., Balogiannis, G. (2013) “AFM Multimode Imaging and Nanoindetation Method For Assessing Collagen Nanoscale Thin Films Heterogeneity” IFMBE Proceedings, 41, 407-410, doi: 10.1007/978-3-319-00846-2_101
- Stylianou, A., Yova, D., Alexandratou, E., Petri, Α. (2013) “Atomic force imaging microscopy investigation of the interaction of ultraviolet radiation with collagen thin films ” Progress in Biomedical Optics and Imaging- Proceedings of SPIE, 8594, art. no. 85940E; doi:10.1117/12.2002460
- Stylianou, A.,Yova, D., Politopoulos, K (2012) “Atomic Force Microscopy Surface Nanocharacterization of UV-Irradiated Collagen Thin Films”, IEEE Proceedings, 12th International Conference on Bioinformatics & Bioengineering (BIBE 2012), art. no. 6399741, pp. 602-607, doi: 10.1109/BIBE.2012.6399741
- Kontomaris, S.V., Stylianou, A., Yova, D., Politopoulos, K (2012) “Mechanical Properties of Collagen Fibrils on Thin Films by Atomic Force Microscopy Nanoindentation”, IEEE Proceedings, 12th International Conference on Bioinformatics & Bioengineering (BIBE 2012), art. no. 6399742, pp. 608-613, doi: 10.1109/BIBE.2012.6399742
- Stylianou, A.,Yova, D., Politopoulos, K. (2012) “Atomic Force Microscopy Quantitative and Qualitative Nanoscale Characterization of Collagen Thin Films”, Proceedings of the 5th Conference on Emerging Technologies in NDT, CRC Press 2012, Chapter 65, 415-420, doi: 10.1201/b11837-75
- Stylianou, A., Politopoulos, K., Yova, D. (2011) “Atomic Force Microscopy Imaging of the Nanoscale Assembly of Type I Collagen on Controlled Polystyrene Particles Surfaces” IFMBE Proceedings, 37, pp. 1058-1061, doi: 10.1007/978-3-642-23508-5_275
- Stylianou, A., Kontomaris S.B., Kyriazi, M., Yova, D.(2010) “Surface characterization of collagen films by atomic force microscopy” IFMBE Proceedings, 29, pp. 612-615, doi: 10.1007/978-3-642-13039-7_154
- Stylianou, A., Kyriazi M, Politopoulos K, Yova D.(2009) “Combined SHG signal information with AFM imaging to assess conformational changes in collagen” IEEE Proceedings, 9th International Conference on Information Technology and Applications in Biomedicine (ITAB 2009), art. no. 5394311, 1-4, doi: 10.1109/ITAB.2009.5394311
Patents
- JD Martin, T Stylianopoulos, F Mpekris, M Panagi, C Voutouri, A Stylianou “Cancer treatment using bosentan in combination with a checkpoint inhibitor” , US Patent App. 18/256,776 (Non-provisional filing on 10/12/2021), https://patents.google.com/patent/US20240024317A1/en
Monographs/Theses
- Stylianou, A (2017) “Ethical and Social Aspects of New Medical Technology” (PhD thesis), Open University of Cyprus (ISBN 978-9963-695-61-4)
- Stylianou, A (2014) “Optical Radiation Effects on Collagen Topology Studied with AFM and SEM ” (PhD thesis), assessed through Greece National Documentation Center and National Technical University Central Library.
- Stylianou, A (2011) “Nanotechnology supported emerging non ionizing medical imaging modalities: The case of OCT and THz” (Master Thesis), The Open University, United Kingdom
- Stylianou, A (2007) “Study of the Production of Second Harmonic Generation from Collagen Structures with Atomic Force Microscopy” (Diploma thesis), assessed through National Technical University Central Library (ID. no. 000112848).
Encyclopedia Entries
- Entry ID: 21308, Category: Biophysics, Title: Collagen Fibrils, Authors: Andreas Stylianou , Stylianos Vasileios Kontomaris, Created Date: 2 April 2022, Author: Andreas Stylianou, https://encyclopedia.pub/entry/21308
- Entry ID: 21307, Category: Biophysics, Title: Collagen D-Band Periodicity, Authors: Andreas Stylianou, Created Date: 2 April 2022, Author: Andreas Stylianou, https://encyclopedia.pub/entry/21307
Conference Presentations (presented by PI)
- Stylianou, A (2025) “Nanomechanical biomarkers for non-communicable diseases”, AFM BioMed Conference 2025, 8th-11th April 2025, Barcelona, Spain (podium)
- Stylianou, A (2024) “AFM-Based nanomechanical biomarkers”, ESB2024, 29th Congress of the European Society of Biomechanics, 30 June- 3 July, 2024, Edinburgh, Scotland (Perspective Talk)
- Stylianou, A (2024) “A linear fit for determining Young’s modulus in very deep spherical indentations”, ESB2024, 29th Congress of the European Society of Biomechanics, 30 June- 3 July, 2024, Edinburgh, Scotland (poster)
- Stylianou, A (2024) “Nanomechanical fingerprints of pulmonary fibrosis”, NANOINBIO 2024, Nanosicences for Life and Materials Sciences, 4th edition, April 14 -20, Le Gosier, Gouadeloupe, French Caribbean, France (podium)
- Stylianou, A., (2023) “AFM-based nanomechanical biomarkers: from cancer to pulmonary fibrosis” EBSA 2023, July 31 – August 4, Stockholm, Sweeden (poster)
- Stylianou, A., (2022) “Nanomechanical assessment of solid tumors” 10th International Multithematic Bio-Medical Congress (IMBMC), 4 – 5 November, Nicosia, Cyprus (poster)
- Stylianou, A., (2022) “Identifying unique nanomechanical signatures as possible biomarkers”, 1st Βιος-SBSCy Conference, 22-23 October, Nicosia, Cyprus (poster)
- Stylianou, A., (2022) “AFM-based nanomechanical biomarkers”, 2022 NanoScientific Forum Europe, 6-7 October, (online, podium)
- Stylianou, A., (2022) “MechanoOptical fingerprints as biomarkers for cancer treatment monitoring”, COMULIS Conference Nicosia, 21 / 9/ 2022, Cyprus (podium)
- Stylianou, A., Polemdiotou, K., Mpekris, F. Stylianopoulos, T. (2022) “Nanomechanical signature of fibrosarcoma: from single cells to tissue level”, ESB2022, 27th Congress of the European Society of Biomechanics, 26-26 June, Porto, Portugal (podium)
- Stylianou, A., Kontomaris, S.V., Malamou, A., (2022) “Determining tip radius in AFM nanoindentation”, ESB2022, 27th Congress of the European Society of Biomechanics, 26-26 June, Porto, Portugal (poster)
- Stylianou, A., Voutouri, C., Mpekris, F., Papouis,A., Polemidiotou, K., Stylianopoulos T., (2022) “Solid tumors nanomechanical fingerprints and collagen-based optical signatures”, Tissue Optics and Photonics II, SPIE Photonics Europe, 3 – 7 April, Strasbourg, France (podium)
- Stylianou, A., ” Atomic force microscopy based nanomechanical biomarkers” 9th International Multithematic Bio-Medical Congress (IMBMC), 18 – 20 November, Nicosia, Cyprus (poster)
- Stylianou, A. (2021) “Nanomechanical and Optical Collagen-based Signatures as Cancer Biomarkers” 7th Freiberg Collagen Symposium September 29 and 30, Germany (online format, invited, podium)
- Stylianou, A. (2021) “Nanomechanical assessment of cancer cells and solid tumors as a mechanical biomarker” ESCHM-ISCH-ISB, symposium Nanomechanical and nanorheological assessments of various diseases, July 4-7, Fukuoka, Japan, (online format, invited, podium)
- Stylianou, A., Mpekris, F., Voutouri, C., Stylianopoulos T., (2021) “Nanomechanical finger-prints of solid tumors as a treatment monitoring biomarker”, 26th Congress of the European Society of Biomechanics, Jully 11-14, Milan, Italy (online format, podium)
- Stylianou, A., Voutouri, C., Mpekris, F., Stylianopoulos T., (2021) “Nanomechanical Signature of sarcomas can be predictive of response to chemotherapy”, SB3C2021 Summer Biomechanics, Bioengineering and Biotransport Conference, June 14-18, USA (virtual, podium)
- Stylianou, A., Voutouri, C., Mpekris, F., Stylianopoulos T., (2021) “Pancreatic Cancer Collagen-Based Optical Signatures”, Polarized light and Optical Angular Momentum for biomedical diagnostics, SPIE BiOS, March 6 – 11, San Francisco, California, USA (online format, podium).
- Stylianou, A. (2019) “Extracellular matrix stiffness and transforming growth factor-ß modulates pancreatic stromal cells’ cytoskeletal remodeling” 10th Annual Symposium ”Physics of Cancer”, 25-27, Leipzig, Germany (podium).
- Stylianou, A. (2018) “Cell and tissue nanomechanical properties in cancer research” 2nd “Current Advances in Cancer Research” Colloquium, 13 December 2018, European University, Nicosia, Cyprus (podium).
- Stylianou, A, Gkretsi, V. and Stylianopoulos, T. (2018) “Pancreatic Cancer Associated Fibroblasts Stiffness and Invasion Properties are Modulated by Transforming Growth Factor-β” 8th World Congress of Biomechanics, 8-12 July, Dublin, Ireland (podium).
- Stylianou, A, Gkretsi, V. and Stylianopoulos, T. (2017) “Normal and Cancer Associated Pancreatic Fibroblasts Shape Modulation is mediated by Transforming Growth Factor β and Matrix Stiffness”, AFM Biomed Conference, 4-8 September 2017, Krakow, Poland (podium).
- Stylianou, A, Stylianopoulos, T. (2016) “Investigation of the effect of Tumor Growth Factor-β on Pancreatic Normal and Cancer Associated Fibroblasts”, Cyprus Researchers’ Night 2016, 30 September, Nicosia, Cyprus (poster).
- Stylianou, A, Gkretsi, V. and Stylianopoulos, T. (2016) “Effects of Tumor Growth Factor β on Pancreatic Cancer Associated Fibroblasts and Fibroblasts”, EUROAFMForum 2016, June, 22-24, 2016, Geneva, Switzerland (podium).
- Stylianou, A., Kontomaris, S.V., Yova, D (2016) “Probing Collagen Nanocharacteristics After Low-Level Red Laser Irradiation” XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016, March 31st–April 2nd 2016, Paphos, Cyprus (podium).
- Kontomaris, S.V., Yova, D, Sambani, k and Stylianou, A. (2016) ” AFM Investigation of the Influence of Red Light Irradiation on Collagen” XIV Mediterranean Conference on Medical and Biological Engineering and Computing 2016, March 31st–April 2nd 2016, Paphos, Cyprus (podium).
- Stylianou, A., Yova, D., (2014) “Optical Radiation Effects on Collagen Topology Studied with AFM and SEM”, Heracleitus II Dissemination Workshop, 22 May 2014, NTUA, Athens, Greece, (poster).
- Stylianou, A., Yova, D., Alexandratou, E. (2013) “Investigation of UV interactions with collagen thin films by using AFM microscopy imaging”, 8th Meeting of Hellenic Society for Biomaterials, 15-16 November 2013, Athens, Greece (podium).
- Stylianou, A., Yova, D., Alexandratou, E. (2013) “Assessing Fibroblasts Response on Collagen Thin Films Nanotopography by Atomic Force Microcopy”, ELEVIT 2013, 5th Panhellenic Conference on Biomedical Technologies, 4-6 April 2013, Athens, Greece (poster).
- Stylianou, A., Yova, D., Alexandratou, Ε., Petri, Α. (2013) “Atomic force imaging microscopy investigation of the interaction of ultraviolet radiation with collagen thin films”, SPIE Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications X, 85940E, part of BIOS 2013, 2-7 February 2013, San Francisco, California, USA (podium).
- Stylianou, A., Yova, D., Politopoulos, K. (2012) “Atomic Force Microscopy Surface Nanocharacterization of UV-Irradiated Collagen Thin Films”, IEEE 12th International Conference on Bioinformatics & Bioengineering, BIBE 2012, 11-13 November 2012, Larnaca, Cyprus (podium).
- Kontomaris, S.V., Stylianou, A., Yova, D., Politopoulos, K (2012) “Mechanical Properties of Collagen Fibrils on Thin Films by Atomic Force Microscopy Nanoindentation”, 12th International Conference on Bioinformatics & Bioengineering, BIBΕ 2012, 11-13 November, 2012, Larnaca, Cyprus (podium).
- Stylianou, A., Kontomaris, S.V. and Yova, D. (2012) “Nanocharacterization of UV-Irradiated Collagen Thin Films by Atomic Force Microscopy Imaging and Nanoindentation”, Fibrous Protein Nanocomposities for Tailored Hybrid Biostructures and Devices-ECI, 8-12 October, 2012, Daios Cove Resort, Crete, Greece (poster).
- Stylianou, A., Yova, D., Petri, A. (2012) “AFM Characterization of Collagen Thin Films for Assessing Fibroblasts Response on Collagen Nanotopography”, 9th International Conference on Nanoscience & Nanotechnologies, 3-6 July, 2012, Thessaloniki, Greece (poster).
- Stylianou, A., Yova, D., Politopoulos, K. (2012) “Development and Atomic Force Microscopy Nanoscale Characterization of Collagen Thin Films for Medical and Biological Applications”, 4th Panhellenic Conference on Biomedical Technologies, International Federation for Medical and Biological Engineering, ELEVIT 2012, 20-21 January 2012, NTUA, Athens, Greece (poster).
- Stylianou, A., Yova, D., Politopoulos, K. (2011) “Collagen Thin Films Development for Biomedical Applications”, 6st Meeting of Hellenic Society for Biomaterials, November 18-19, Athens, Greece (podium).
- Stylianou, A., Yova, D., Politopoulos, K. (2012) “Atomic Force Microscopy Quantitative and Qualitative Nanoscale Characterization of Collagen Thin Films”, Emerging Technologies in Non-Destructive Testing V- 5th Conference on Emerging Technologies in NDT, 19-21 September 2011, Ioannina, Greece (podium).
- Stylianou, A., Politopoulos, K., Yova, D. (2011) “Atomic Force Microscopy Imaging of the Nanoscale Assembly of Type I Collagen on Controlled Polystyrene Particles Surfaces”, 5th European IFMBE Conference, EMBEC 2011, 14-18 September 2011, Budapest, Hungary (poster).
- Stylianou, A., Kontomaris, S.B., Kyriazi, M., Yova D. (2010) “Surface characterization of collagen films by atomic force microscopy”, 12th Mediterranean Conference on Medical and Biological Engineering and Computing, MEDICON 2010, 27-30 May 2010, Chalkidiki, Greece (podium).
- Stylianou, A., Kyriazi, M., Politopoulos, K., Yova, D. (2009) “Combined SHG signal information with AFM imaging to assess conformational changes in collagen”, 9th International Conference on Information Technology and Applications in Biomedicine, IEEE ITAB 2009, 4-7 November 2009, Larnaca, Cyprus (poster).
- Stylianou, A., Kyriazi, M., Politopoulos, K., Yova, D. (2009) “Second harmonic generation and atomic force microscopy study of collagen thin films” 4th International Graduate Summer School: Biophotonics ′09, 6-13 June 2009, Ven, Sweden (poster).
- Stylianou, A., Kyriazi, M., Yova, D. (2009) “Study of the influence of physicochemical factors in the creation of thin collagen films with atomic force microscopy”, 1st Meeting Hellenic Society for Biomaterials & H.A.O.S.T, 27-29 November 2009, Athens, Greece (podium).
- Stylianou, A., Kyriazi, M., Yova, D. (2009) “Study of collagen type I with AFM”, 3rd Meeting of Hellenic Society for Biomaterials, 21-22 November 2008, Athens, Greece (poster).
Conference Presentations (presented by other)
- Kontomaris, S.V., Stylianou, A (2025) “Overcoming challenges in the AFM mechanical nanocharacterization of soft heterogeneous biological materials”, AFM BioMed Conference 2025, 8th-11th April 2025, Barcelona, Spain (podium)
- Polemidiotou, K., Stylianou, A., (2022) “Collagen content and stiffness alter the cytoskeleton and nanomechanical properties of sarcoma cells”, 1st Βιος-SBSCy Conference, 22-23 October, Nicosia, Cyprus (poster)
- Polemidiotou, K., Stylianou, A., (2022) “Collagen content and stiffness alter the cytoskeleton and nanomechanical properties of sarcoma cells”, 2022 NaoScientific Forum Europe, 6-7 October, (online, podium)
- Polemidiotou, K., Stylianou, A., (2021) “Collagen content and stiffness cause cytoskeletal remodelling in sarcoma cells” 9th International Multithematic Bio-Medical Congress (IMBMC), 18 – 20 November, Nicosia, Cyprus (podium)
- Stavrou, M., Papaparaskeva, G., Stathis, A., Stylianou, A., Turcu, R., Krasia-Christoforou, T. and Couri, S. (2021) “Synthesis, Characterization and Nonlinear Optical Response of Polyelectrolyte-Stabilised Copper Hydroxide and Copper Oxide Colloidal Nanohybrids”, XXXV Panhellenic Conference on Solid State Physics and Material Science, 26-29 September 2021, Athens, Greece (virtual, poster).
- Kontomaris, S.V., Malamou, A., Stylianou, A. (2021) “Indentation method using spherical indenters: Determing the errors while using Hertz equation”, 26th Congress of the European Society of Biomechanics, Jully 11-14, Milan, Italy (online format, poster)
- Kapnisis, K., Stylianou, A., Kokkinidou, D., Prokopi, M., Anayiotos, A. (2021) “Lymphocyte nanomechanics in response to strent implantation”, SB3C2021 Summer Biomechanics, Bioengineering and Biotransport Conference, June 14-18, USA (virtual, podium)
- Louca, M., Stylianou A., Minia, A., Pliaka, V., Alexopoulos, L., Gkretsi, V., and Stylianopoulos, T. (2018) “Opposing effects of RSU-1 silencing in glioma cell invasion depending on their aggressiveness” 6th International Multithematic Bio-Medical Congress (IMBMC), Bio-Medical Scientific Cyprus (BSC), 15-17 November, 2018, Nicosia, Cyprus (1st poster award) (poster).
- Louca, M., Stylianou, A., Gkretsi, V., and Stylianopoulos, T. (2018), “The Role of RSU-1 in Glioma Cells”, 25th Biennial Congress of the European Association for Cancer Research (EACR25) 30 June – 03 July, Amsterdam (poster).
- Gkretsi V., Louca M., Stylianou A., Minadakis G., Spyrou G., and Stylianopoulos T. (2018) “Inhibition of breast cancer cell invasion by Ras suppressor-1 (RSU-1) silencing is reversed by Growth Differentiation Factor-15 (GDF-15)”, 7th International Conference of the Cyprus Society of Human Genetics, December 7th 2018, Nicosia, Cyprus (podium).
- Gkretsi V., Stylianou A., Louca M., and Stylianopoulos T. (2017) “Identification of Ras Suppressor-1 (RSU-1) and Vasodilator-Stimulated Phosphoprotein (VASP) as potential breast cancer metastasis biomarkers using a three-dimensional in vitro approach”, 7th International Conference on Tumor-Host Interaction and Angiogenesis, 25-28 June, 2017, Monte Verità, Ascona, Switzerland (poster).
- Gkretsi, V., Stylianou, A, Stylianopoulos, T. (2016) “Targeting breast cancer metastasis: A bioengineering approach”, 21st World Congress on Advances in Oncology & 19th International Symposium of Molecular Medicine, 6-8 October, 2016, Athens, Greece (podium).
- Stylianou, A., Kontomaris, S.V., Yova, D., Balogiannis, G. (2013) “AFM Multimode Imaging and Nanoindetation Method For Assessing Collagen Nanoscale Thin Films Heterogeneity” XIII Mediterranean Conference on Medical and Biological Engineering and Computing – MEDICON 2013, 25-28 September 2013, Sevilla, Spain (podium).
- Kontomaris, S. V., Stylianou, Α., Yova, D. (2013) “Investigation of collagen thin films mechanical heterogeneity in nanoscale using AFM”, 8th Meeting of Hellenic Society for Biomaterials, 15- 16 November 2013, Athens, Greece (podium).
- Kontomaris, S.V., Yova, D., Stylianou, A., Sampani, K., Balogiannis, G., Politopoulos, K. (2013) “Mechanical Heterogeneity of Collagen Fibrils”, 5th Panhellenic Conference on Biomedical Technologies, ELEVIT 2013, 4-6 April 2013, Athens, Greece (poster).